Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics.

Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner

Evaluating indoor positioning systems in a shopping mall : the lessons learned from the IPIN 2018 competition

The Indoor Positioning and Indoor Navigation (IPIN) conference holds an annual competition in which indoor localization systems from different research groups worldwide are evaluated empirically. The objective of this competition is to establish a systematic evaluation methodology with rigorous metrics both for real-time (on-site) and post-processing (off-site) situations, in a realistic environment unfamiliar to the prototype developers. For the IPIN 2018 conference, this competition was held on September 22nd, 2018, in Atlantis, a large shopping mall in Nantes (France). Four competition tracks (two on-site and two off-site) were designed. They consisted of several 1 km routes traversing several floors of the mall. Along these paths, 180 points were topographically surveyed with a 10 cm accuracy, to serve as ground truth landmarks, combining theodolite measurements, differential global navigation satellite system (GNSS) and 3D scanner systems. 34 teams effectively competed. The accuracy score corresponds to the third quartile (75th percentile) of an error metric that combines the horizontal positioning error and the floor detection. The best results for the on-site tracks showed an accuracy score of 11.70 m (Track 1) and 5.50 m (Track 2), while the best results for the off-site tracks showed an accuracy score of 0.90 m (Track 3) and 1.30 m (Track 4). These results showed that it is possible to obtain high accuracy indoor positioning solutions in large, realistic environments using wearable light-weight sensors without deploying any beacon. This paper describes the organization work of the tracks, analyzes the methodology used to quantify the results, reviews the lessons learned from the competition and discusses its future

The IPIN 2019 Indoor Localisation Competition—Description and Results

IPIN 2019 Competition, sixth in a series of IPIN competitions, was held at the CNR Research Area of Pisa (IT), integrated into the program of the IPIN 2019 Conference. It included two on-site real-time Tracks and three off-site Tracks. The four Tracks presented in this paper were set in the same environment, made of two buildings close together for a total usable area of 1000 m 2 outdoors and and 6000 m 2 indoors over three floors, with a total path length exceeding 500 m. IPIN competitions, based on the EvAAL framework, have aimed at comparing the accuracy performance of personal positioning systems in fair and realistic conditions: past editions of the competition were carried in big conference settings, university campuses and a shopping mall. Positioning accuracy is computed while the person carrying the system under test walks at normal walking speed, uses lifts and goes up and down stairs or briefly stops at given points. Results presented here are a showcase of state-of-the-art systems tested side by side in real-world settings as part of the on-site real-time competition Tracks. Results for off-site Tracks allow a detailed and reproducible comparison of the most recent positioning and tracking algorithms in the same environment as the on-site Tracks

Label-free cell separation and sorting in microfluidic systems

Cell separation and sorting are essential steps in cell biology research and in many diagnostic and therapeutic methods. Recently, there has been interest in methods which avoid the use of biochemical labels; numerous intrinsic biomarkers have been explored to identify cells including size, electrical polarizability, and hydrodynamic properties. This review highlights microfluidic techniques used for label-free discrimination and fractionation of cell populations. Microfluidic systems have been adopted to precisely handle single cells and interface with other tools for biochemical analysis. We analyzed many of these techniques, detailing their mode of separation, while concentrating on recent developments and evaluating their prospects for application. Furthermore, this was done from a perspective where inertial effects are considered important and general performance metrics were proposed which would ease comparison of reported technologies. Lastly, we assess the current state of these technologies and suggest directions which may make them more accessible

MagIO: Magnetic Field Strength Based Indoor- Outdoor Detection with a Commercial Smartphone

A wide range of localization techniques has been proposed recently that leverage smartphone sensors. Context awareness serves as the backbone of these localization techniques, which helps them to shift the localization technologies to improve efficiency and energy utilization. Indoor-outdoor (IO) context sensing plays a vital role for such systems, which serve both indoor and outdoor localization. IO systems work with collaborative technologies including the Global Positioning System (GPS), cellular tower signals, Wi-Fi, Bluetooth and a variety of smartphone sensors. GPS- and Wi-Fi-based systems are power hungry, and their accuracy is severed by limiting factors like multipath, shadowing, etc. On the other hand, various built-in smartphone sensors can be deployed for environmental sensing. Although these sensors can play a crucial role, yet they are very less studied. This research aims at investigating the use of ambient magnetic field data alone from a smartphone for IO detection. The research first investigates the feasibility of utilizing magnetic field data alone for IO detection and then extracts different features suitable for IO detection to be used in machine learning-based classifiers to discriminate between indoor and outdoor environments. The experiments are performed at three different places including a subway station, a shopping mall and Yeungnam University (YU), Korea. The training data are collected from one spot of the campus, and testing is performed with data from various locations of the above-mentioned places. The experiment involves Samsung Galaxy S8, LG G6 and Samsung Galaxy Round smartphones. The results show that the magnetic data from smartphone magnetic sensor embody enough information and can discriminate the indoor environment from the outdoor environment. Naive Bayes (NB) outperforms with a classification accuracy of 83.26%, as against Support vector machines (SVM), random induction (RI), gradient boosting machines (GBM), random forest (RF), k-nearest neighbor (kNN) and decision trees (DT), whose accuracies are 67.21%, 73.38%, 73.40%, 78.59%, 69.53% and 68.60%, respectively. kNN, SVM and DT do not perform well when noisy data are used for classification. Additionally, other dynamic scenarios affect the attitude of magnetic data and degrade the performance of SVM, RI and GBM. NB and RF prove to be more noise tolerant and environment adaptable and perform very well in dynamic scenarios. Keeping in view the performance of these classifiers, an ensemble-based stacking scheme is presented, which utilizes DT and RI as the base learners and naive Bayes as the ensemble classifier. This approach is able to achieve an accuracy of 85.30% using the magnetic data of the smartphone magnetic sensor. Moreover, with an increase in training data, the accuracy of the stacking scheme can be elevated by 0.83%. The performance of the proposed approach is compared with GPS-, Wi-Fi- and light sensor-based IO detection

BLocate: A Building Identification Scheme in GPS Denied Environments Using Smartphone Sensors

Indoor localization systems assume that the user’s current building is known by the GPS (Global Positioning System). However, such assumptions do not hold true in GPS denied environments or where the GPS cannot determine the user’s definite location. We present a novel solution to identify the building where the user is present now. The proposed building identification method works on the pervasive magnetic field using a smartphone. The accelerometer data determines the user’s activity of being stationary or walking. An Artificial Neural Network is used to identify the user’s activities and it shows good results. The magnetometer data is used to identify the user’s current building using the fingerprinting approach. Contrary to a traditional fingerprinting approach which stores intensity values, we utilize the patterns formed by the magnetic field strength in the form of a Binary Grid (BG). The BG approach overcomes the limitation of Dynamic Time Warping (DTW) whose performance is degraded when the magnitude of the magnetic data is changed. The experiments are performed with Samsung Galaxy S8 for eight various buildings with different altitudes and number of floors in Yeungnam University, Korea. The results demonstrate that the proposed building identification method can potentially be deployed for building identification. The precision, UAR (Unweighted Average Recall), F score, and Cohen’s Kappa values are used to determine the performance of the proposed system. The proposed systems shows very promising results. The system operates without any aid from any infrastructure dependent technologies like GPS or WiFi. Furthermore, we performed many experiments to investigate the impact of isolated points data to build fingerprint database on system’s accuracy with 1 m and 2 m distance. Results illustrate that by trading off a minor accuracy, survey labor can be reduced by 50 percent

Smartphone Sensor Based Indoor Positioning: Current Status, Opportunities, and Future Challenges

The last two decades have witnessed a rich variety of indoor positioning and localization research. Starting with Microsoft Research pioneering the fingerprint approach based RADAR, MIT’s Cricket, and then moving towards beacon-based localization are few among many others. In parallel, researchers looked into other appealing and promising technologies like radio frequency identification, ultra-wideband, infrared, and visible light-based systems. However, the proliferation of smartphones over the past few years revolutionized and reshaped indoor localization towards new horizons. The deployment of MEMS sensors in modern smartphones have initiated new opportunities and challenges for the industry and academia alike. Additionally, the demands and potential of location-based services compelled the researchers to look into more robust, accurate, smartphone deployable, and context-aware location sensing. This study presents a comprehensive review of the approaches that make use of data from one or more sensors to estimate the user’s indoor location. By analyzing the approaches leveraged on smartphone sensors, it discusses the associated challenges of such approaches and points out the areas that need considerable research to overcome their limitations

Recent Advancements in Indoor Positioning and Localization

The current era celebrates the rise of mobile devices, most of which are mobile phones [...